What question did this study set out to answer?

To identify the structural requirements and enzymatic mechanisms responsible for the TNF-induced proteolytic degradation of IκBα.

May 1, 1995Molecular and Cellular BiologyOpen Access

Inducible Degradation of IκBα In Vitro and In Vivo Requires the Acidic C-Terminal Domain of the Protein

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To identify the structural requirements and enzymatic mechanisms responsible for the TNF-induced proteolytic degradation of IκBα.
Developed a cell-free degradation assay using cell lysates prepared from TNF-stimulated and unstimulated cells incubated with recombinant IκBα.
Evaluated protease inhibitor sensitivity to characterize the enzyme responsible for IκBα degradation.
Tested mutated forms of IκBα, including NF-κB binding mutants and a 61-amino-acid C-terminal deletion mutant, in cell-free systems and in transfected COS-7 cells.
Recombinant IκBα was selectively degraded in lysates from TNF-exposed cells but remained stable in untreated cell extracts, with inhibitor profiles implicating a serine protease.
Mutant IκBα unable to bind NF-κB degraded efficiently, whereas deletion of the acidic C-terminal 61 amino acids blocked degradation both in vitro and in vivo in COS-7 cells.

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Implication

In vitro and in vivo cell assay demonstrates that IκBα degradation requires its acidic C-terminal domain, indicating a critical structural region for TNF-induced immune signaling.

Cite This Study

Rodríguez et al. (1995) studied this question.

synapsesocial.com/papers/6a109b271406dce28afcc579 https://doi.org/10.1128/mcb.15.5.2413

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Implication

In vitro and in vivo cell assay demonstrates that IκBα degradation requires its acidic C-terminal domain, indicating a critical structural region for TNF-induced immune signaling.

Inducible Degradation of IκBα In Vitro and In Vivo Requires the Acidic C-Terminal Domain of the Protein

Key Points

Discussion

Implication

Cite This Study

Inducible Degradation of IκBα In Vitro and In Vivo Requires the Acidic C-Terminal Domain of the Protein

Key Points

Discussion

Implication

Cite This Study

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