Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
August 1, 1986Molecular and Cellular Biology

Redesign of Retrovirus Packaging Cell Lines To Avoid Recombination Leading to Helper Virus Production

View Full Paper
Ask AI
Bookmark
Share

Authors

AMA. Dusty MillerBrigham Young UniversityCBCarol ButtimoreCape Town HVTN Immunology Laboratory / Hutchinson Centre Research Institute of South Africa

Discussion

Loading...

Member takes

Overview

Randomized trial redesigns retrovirus packaging lines to eliminate helper virus production, suggesting a safer gene transfer method.

Key Points

  • The aim is to redesign retrovirus packaging cell lines to prevent the production of helper viruses, ensuring safer gene transfer studies.
  • Constructed packaging cell lines with alterations in helper virus DNA including deletion of the packaging signal.
  • Tested various retroviral vectors to assess helper virus production from the new packaging cells.
  • Retained essential features of previous packaging lines while ensuring no helper virus was produced.
  • The new packaging cell lines did not yield helper virus after vector introduction.
  • No transfer of packaging function was detected, confirming the redesign's effectiveness.

Cite This Study

Miller et al. (1986) studied this question.

synapsesocial.com/papers/6a10cf9839dd87f6d0ee5fcchttps://doi.org/10.1128/mcb.6.8.2895-2902.1986
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Avian oncovirus mutant (SE21Q1b) deficient in genomic RNA: characterization of a deletion in the provirus1980 · 117 citations
  2. 2Generation of helper-free amphotropic retroviruses that transduce a dominant-acting, methotrexate-resistant dihydrofolate reductase gene.1985 · 332 citations
  3. 3A transmissible retrovirus expressing human hypoxanthine phosphoribosyltransferase (HPRT): gene transfer into cells obtained from humans deficient in HPRT.1983 · 211 citations
  4. 4Amphotropic retrovirus vector system for human cell gene transfer.1984 · 105 citations