Protein Synthesis Directed by Encephalomyocarditis Virus RNA: Properties of a Transfer RNA-Dependent System
Population
Ascites tumor cell extracts
Comparison
Addition of exogenous tRNA vs No exogenous tRNA or different sources of tRNA
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Hypothesis-generating for species-specific tRNA in viral translation; leaves open applicability to mammalian systems in vivo.
Structured PICO
P
Population
Ascites tumor cell extracts
I
Intervention
Addition of exogenous tRNA (from ascites tumor cells, rat liver, yeast, or E. coli)
C
Comparator
No exogenous tRNA or different sources of tRNA
O
Outcome
Amino acid incorporation (protein synthesis)surrogate
Demonstrates that viral RNA translation in ascites tumor cell extracts is dependent on specific exogenous tRNA sources, highlighting translational barriers between species.
Cite This Study
Aviv et al. (1971) studied this question.