Demonstrates that in vitro translation of encephalomyocarditis virus RNA in a reticulocyte lysate system yields an active proteolytic enzyme responsible for processing primary translation products.
In contrast to other cell-free translation systems, the mRNA-dependent reticulocyte lysate can translate encephalomyocarditis virus RNA efficiently and completely when supplemented with heterologous tRNA. Cleavage of the nascent polypeptide chain occurs, and one of the translation products appears to be a specific proteolytic enzyme which correctly processes the primary products. The identity of the proteins made in vitro was verified by comparison with infected cell proteins on dodecylsulphate/polyacrylamide gels, and by mapping their coding sequences on the viral genome.
Hugh R.B. Pelham (Sat,) studied this question.