Role of transiently altered sarcolemmal membrane permeability and basic fibroblast growth factor release in the hypertrophic response of adult rat ventricular myocytes to increased mechanical activity in vitro.
Population
Primary isolates of adult rat ventricular myocytes maintained in serum-free, defined medium
Comparison
Continual pacing at 3 Hz for up to 5 days vs Nonpaced control cells
Design
Preclinical
Follow-up
up to 5 days
Authors
Discussion
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Member takes
Implication
No immediate clinical implications; leaves open bFGF as target in human pacing-induced hypertrophy.
Key Points
- This research aims to investigate how increased mechanical activity affects sarcolemmal membrane permeability and bFGF release in adult rat ventricular myocytes.
- Adult rat ventricular myocytes were isolated and maintained in serum-free medium while being paced at 3 Hz for up to 5 days.
- Hypertrophic responses were assessed by measuring phenylalanine incorporation, protein content, and cell size.
- The release of bFGF was measured using ELISA, and membrane permeability was examined using fluorescein-labeled dextran.
- Paced myocytes showed a significant increase in phenylalanine incorporation, total cellular protein content, and cell size compared to nonpaced controls.
- Conditioned medium from paced cells had higher levels of bFGF and mitogenic activity for 3T3 cells, which was reduced by anti-bFGF antibodies.
- Paced myocytes demonstrated a significantly higher uptake of fluorescein-labeled dextran, indicating increased sarcolemmal permeability.
Structured PICO
P
Population
Primary isolates of adult rat ventricular myocytes maintained in serum-free, defined medium
I
Intervention
Continual pacing at 3 Hz for up to 5 days
C
Comparator
Nonpaced control cells
O
Outcome
Hypertrophic response (rate of phenylalanine incorporation, total cellular protein content, and cell size)surrogate
Increased mechanical activity induces a hypertrophic response in cardiac myocytes through transient sarcolemmal membrane disruptions and release of cytosolic bFGF.
Cite This Study
Kaye et al. (1996) studied this question.