A recombinant plasmid from which an infectious adeno-associated virus genome can be excised in vitro and its use to study viral replication
Population
Adenovirus-infected 293 cells and recombinant adeno-associated virus (AAV) plasmids
Comparison
Excision of viral DNA from the vector with PvuII… vs Unexcised viral DNA
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Does not support changes to AAV vector design; leaves open in vivo validation of excision and strand infectivity.
Structured PICO
P
Population
Adenovirus-infected 293 cells and recombinant adeno-associated virus (AAV) plasmids
I
Intervention
Excision of viral DNA from the vector with PvuII before transfection
C
Comparator
Unexcised viral DNA (transfection of the intact recombinant plasmid)
O
Outcome
Viral DNA replication efficiency
The development of a novel recombinant AAV plasmid allowing in vitro excision of the viral genome demonstrated that pre-excision enhances replication efficiency and that both viral DNA strands are infectious.
Cite This Study
Samulski et al. (1987) studied this question.