Left ventricular assist device bioinformatics identify possible hubgenes and regulatory networks involved in the myocardium of patients with left ventricular assist device

Figures

• 

  FIGURE 1. Venn diagram of differentially expressed genes from four transcriptome datasets.

• 

  FIGURE 2. The volcano plots of differentially expressed genes in four datasets. Red indicates genes with higher levels of expression, blue indicates genes with lower levels of expression, and gray indicates genes with no differential expression based on the criteria of P 0.05 and |logFC| 1.0, respectively. P.adj, adjusted P value.

• 

  FIGURE 3. Heatmaps of differentially expressed genes in the four transcriptome datasets showed the hierarchical clustering characteristics of gene transcription changes in a cluster analysis of different groups. VAD, ventricular assist device; ICM, ischemic cardiomyopathy; DCM, dilated cardiomyopathy.

• 

  FIGURE 4. The GO enrichment analysis and KEGG pathway of differentially expressed genes in HF patients before and after LVAD implantation. The color depth of nodes refers to the corrected P-value of ontologies. The size of nodes refers to the numbers of genes that are involved in the ontologies. P < 0.01 was considered statistically significant.

• 

  FIGURE 5. A scattergram composed of GO description and associated genes. Blue nodes represent GO terms, red nodes represent differentially expressed genes (DEG), and the lines indicate that a DEG has a corresponding GO annotation. The node size is consistent with the number of counts. 1. Neurotransmitter binding, 2. postsynaptic membrane, 3. neuron spine, 4. dendritic spine, 5. regulation of calcium ion transport, 6. response to molecule of bacterial origin, 7. response to lipopolysaccharide, 8. osteoclast differentiation, 9. receptor ligand activity, 10. cytokine activity, 11. cytokine-cytokine receptor interaction, 12. non-small cell lung cancer.

• 

  FIGURE 6. A network panorama of protein-protein interaction composed of all differentially expressed genes of heart failure patients before and after LVAD implantation. Lines between nodes represent interactions between genes.

• 

  FIGURE 7. The key module identified based on MCODE (Molecular Complex Detection) plug-in analysis. The gene of CXCL12 highlighted in yellow is the seed gene of the whole module.