Interactions between KCNQ1 and KCNE1 cause pore constriction and energetic barriers in the ion-permeation pathway, which are further pronounced upon binding of Chromanol 293B and its derivatives.
Molecular dynamics simulations reveal that KCNE1 interaction and ligand binding increase energetic barriers for potassium permeation in the KCNQ1 channel, providing insights into potential drug cardiotoxicity mechanisms.
The voltage-gated KCNQ1 potassium ion channel interacts with the type I transmembrane protein minK (KCNE1) to generate the slow delayed rectifier (IKs) current in the heart. Mutations in these transmembrane proteins have been linked with several heart-related issues, including long QT syndromes (LQTS), congenital atrial fibrillation, and short QT syndrome. Off-target interactions of several drugs with that of KCNQ1/KCNE1 ion channel complex have been known to cause fatal cardiac irregularities. Thus, KCNQ1/KCNE1 remains an important avenue for drug-design and discovery research. In this work, we present the structural and mechanistic details of potassium ion permeation through an open KCNQ1 structural model using the combined molecular dynamics and steered molecular dynamics simulations. We discuss the processes and key residues involved in the permeation of a potassium ion through the KCNQ1 ion channel, and how the ion permeation is affected by (i) the KCNQ1-KCNE1 interactions and (ii) the binding of chromanol 293B ligand and its derivatives into the complex. The results reveal that interactions between KCNQ1 with KCNE1 causes a pore constriction in the former, which in-turn forms small energetic barriers in the ion-permeation pathway. These findings correlate with the previous experimental reports that interactions of KCNE1 dramatically slows the activation of KCNQ1. Upon ligand-binding onto the complex, the energy-barriers along ion permeation path are more pronounced, as expected, therefore, requiring higher force in our steered-MD simulations. Nevertheless, pulling the ion when a weak blocker is bound to the channel does not necessitate high force in SMD. This indicates that our SMD simulations have been able to discern between strong and week blockers and reveal their influence on potassium ion permeation. The findings presented here will have some implications in understanding the potential off-target interactions of the drugs with the KCNQ1/KCNE1 channel that lead to cardiotoxic effects.
Hasani et al. (Wed,) conducted a other in Long QT syndrome (contextual). KCNE1 interaction and Chromanol 293B binding vs. KCNQ1 alone was evaluated on Force required for potassium ion permeation. Interactions between KCNQ1 and KCNE1 cause pore constriction and energetic barriers in the ion-permeation pathway, which are further pronounced upon binding of Chromanol 293B and its derivatives.