Key result
In cat atrial myocytes, subsarcolemmal Ca2+ sparks from junctional SR were 3-4 times more frequent than central sparks from non-junctional SR, driven by surface membrane Ca2+ channel association.
Population
Cat atrial myocytes
Comparison
Experimental conditions including caffeine… vs Control conditions (unstimulated intact cells)
Design
Preclinical
Authors
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May inform atrial Ca2+ spark mechanisms in animals; leaves open translation to human AF pathophysiology.
In cat atrial myocytes, the higher frequency of spontaneous subsarcolemmal Ca2+ sparks compared to central sparks is driven by the structural arrangement of the sarcolemma and junctional SR membrane rather than intrinsic differences in ryanodine receptor clusters.
Sheehan et al. (2006) studied Cat atrial myocytes (basic science). Caffeine, verapamil, extracellular Ca2+ removal, and saponin permeabilization vs. Control conditions was evaluated on Spontaneous Ca2+ spark frequency and properties from junctional and non-junctional sarcoplasmic reticulum. In cat atrial myocytes, subsarcolemmal Ca2+ sparks from junctional SR were 3-4 times more frequent than central sparks from non-junctional SR, driven by surface membrane Ca2+ channel association.
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