What question did this study set out to answer?

Determine the molecular mechanism of platelet adhesion to plasma proteins and characterize the role of glycoprotein IIb/IIIa in recognizing the Arg-Gly-Asp sequence.

March 28, 1986Science

Platelet Membrane Glycoprotein IIb/IIIa: Member of a Family of Arg-Gly-Asp—Specific Adhesion Receptors

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Authors

RPRobert PytelaSan Francisco General Hospital MPMichael D. PierschbacherAmerican Life Science Pharmaceuticals (United States)MGMark H. GinsbergRutgers, The State University of New Jersey

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Implication

Biochemical analysis demonstrates broad adhesive protein binding by glycoprotein IIb/IIIa in platelets, indicating a shared family of Arg-Gly-Asp receptors.

Key Points

Determine the molecular mechanism of platelet adhesion to plasma proteins and characterize the role of glycoprotein IIb/IIIa in recognizing the Arg-Gly-Asp sequence.
Applied an affinity matrix composed of an insolubilized Arg-Gly-Asp heptapeptide to isolate selective membrane proteins from platelet detergent extracts.
Reconstituted purified glycoprotein IIb/IIIa into liposome membranes to evaluate adhesion to surfaces coated with fibrinogen, fibronectin, vitronectin, thrombospondin, or albumin.
The insolubilized Arg-Gly-Asp peptide matrix selectively isolated platelet membrane glycoprotein IIb/IIIa from detergent extracts.
Reconstituted liposomes containing glycoprotein IIb/IIIa bound specifically to fibrinogen, fibronectin, and vitronectin, but showed no binding to thrombospondin or albumin.
Glycoprotein IIb/IIIa demonstrated broader ligand specificity than previously identified fibronectin and vitronectin receptors while sharing recognition of the Arg-Gly-Asp motif.

Cite This Study

Pytela et al. (1986) studied this question.

synapsesocial.com/papers/6a12656ef7bd4f5c7da63d85 https://doi.org/10.1126/science.2420006

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Implication

Biochemical analysis demonstrates broad adhesive protein binding by glycoprotein IIb/IIIa in platelets, indicating a shared family of Arg-Gly-Asp receptors.

Platelet Membrane Glycoprotein IIb/IIIa: Member of a Family of Arg-Gly-Asp—Specific Adhesion Receptors

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Member takes

Implication

Key Points

Cite This Study

Platelet Membrane Glycoprotein IIb/IIIa: Member of a Family of Arg-Gly-Asp—Specific Adhesion Receptors

Discussion

Member takes

Implication

Key Points

Cite This Study

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