Lung mucosal and systemic responses at single-cell resolution in an aerosolized Mycobacterium bovis BCG human challenge model

T cells and preferentially localize to the lung mucosa than newly expanded clones. Public expanded clones in the lung mucosa are validated as mycobacterial antigen-reactive using reporter T cells. These findings provide insights into mucosal and systemic immunity post-mycobacterial infection, informing TB vaccine design.