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May 24, 2026BiomoleculesOpen Access

Transcriptome Profiling of Induced Sputum Identified Upregulated TNF-α/NF-κB Signalling and Downregulated Mitochondrial Respiratory Chain Function in Post-Infectious Bronchiolitis Obliterans

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Why the study?

Post-infectious bronchiolitis obliterans leads to persistent inflammation and fibrotic changes in small airways, but its underlying molecular mechanisms remain to be clarified.

Does transcriptome profiling of induced sputum identify differentially expressed genes and altered signaling pathways in patients with post-infectious bronchiolitis obliterans compared to healthy controls?

Population

Nine patients with PiBO and eight healthy controls

Comparison

PiBO patients vs healthy controls

Design

Case-control study

Key result

Transcriptome analysis of sputum cells from PiBO patients revealed significant upregulation of TNFα-dependent NFκB signalling and downregulation of oxidative phosphorylation versus healthy controls.

Authors

SJSilvija P. JerkicKNKaren NaegeleLGLucia Gronau

Discussion

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Overview

May highlight TNFα/NF-κB targets for PiBO therapy; leaves open clinical utility pending prospective trials.

Key Points

  • This study aims to explore the gene expression changes in sputum from patients with post-infectious bronchiolitis obliterans (PiBO) compared to healthy controls.
  • Analyzed gene expression in sputum cells from 9 PiBO patients and 8 healthy controls.
  • Conducted multiplex immunohistochemistry and mRNA sequencing.
  • Performed KEGG pathway enrichment analysis to evaluate biological targets.
  • PiBO patients showed significantly reduced lung function parameters and increased neutrophil counts.
  • Transcriptome analysis revealed significant upregulation of TNFα-dependent NFκB signaling pathway and downregulation of oxidative phosphorylation.
  • Expression of differentially expressed genes (DEGs) correlated significantly with FEV1 lung function.

Study Design

Type

Case-Control (n=17)

Structured PICO

Does transcriptome profiling of induced sputum identify differentially expressed genes and altered signaling pathways in patients with post-infectious bronchiolitis obliterans compared to healthy controls?

P
Population
9 patients with post-infectious bronchiolitis obliterans (PiBO) and 8 healthy controls (total n=17).
I
Intervention
Transcriptome profiling (mRNA sequencing/MACE-Seq) and multiplex immunohistochemistry (mIHC) of induced sputum cells.
C
Comparator
Healthy controls (n=8).
O
Outcome
Differentially expressed genes (DEGs) in sputum cells.surrogate

Transcriptome profiling of sputum cells in PiBO patients reveals a shift in macrophage polarization, upregulated TNF-α/NF-κB signaling, and downregulated mitochondrial function, which correlate with lung function decline.

Cite This Study

Jerkic et al. (2026) conducted a case-control in Post-infectious bronchiolitis obliterans (PiBO) (n=17). Transcriptome profiling of induced sputum vs. Healthy controls was evaluated on Differentially expressed genes (DEGs) in sputum cells. Transcriptome analysis of sputum cells from PiBO patients revealed significant upregulation of TNFα-dependent NFκB signalling and downregulation of oxidative phosphorylation versus healthy controls.

synapsesocial.com/papers/6a1295e248a0ea166567235dhttps://doi.org/10.3390/biom16050745
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1TGFβ1 and POSTN as biomarkers of postinfectious bronchiolitis obliterans and asthma in children2022 · 8 citations
  2. 2Integrated multiomics reveals a clinically relevant CXCL8-centric protein network in pediatric postinfectious bronchiolitis obliterans2026
  3. 3A68-09 The Tgf-β1/smad Pathway Mediates Macrophage-myofibroblast Transition in Post-mycoplasma Pneumoniae Pneumonia Obliterative Bronchiolitis2026
  4. 4B105-10 Non-Linear Dysanaptic Lung Growth in Patients With Post-Infectious Bronchiolitis Obliterans2026
  5. 5Pathogenesis and Risk Factors of Post-Infectious Bronchiolitis Obliterans in Children: A Focus on Adenovirus and Mycoplasma Infections2026