What question did this study set out to answer?

To investigate the molecular mechanisms by which the helix-loop-helix inhibitor Id-1 controls mammary epithelial cell differentiation, invasion, and aggressive cancer phenotypes.

August 1, 1998Molecular and Cellular BiologyOpen Access

A Novel Pathway for Mammary Epithelial Cell Invasion Induced by the Helix-Loop-Helix Protein Id-1

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Authors

PDPierre‐Yves DesprezCalifornia Pacific Medical Center CLClaudia Qiao LinGeron (United States)NTNicole ThomassetUniversité Claude Bernard Lyon 1

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Implication

In vitro and in vivo models show Id-1 induces metalloproteinase-driven cell invasion, suggesting a regulatory mechanism in mammary remodeling and aggressive breast cancer.

Key Points

To investigate the molecular mechanisms by which the helix-loop-helix inhibitor Id-1 controls mammary epithelial cell differentiation, invasion, and aggressive cancer phenotypes.
Engineered murine mammary epithelial cells (SCp2) to constitutively express Id-1 and evaluated differentiation, anchorage dependence, and basement membrane invasion.
Assessed in vivo tumorigenicity of Id-1-expressing SCp2 cells via injection into nude mice.
Analyzed conditioned medium for secreted proteinases using gelatin zymography and metalloproteinase inhibitors, and examined expression during mammary involution and across human breast cancer cell lines.
Constitutive Id-1 expression blocked functional differentiation, induced slow basement membrane invasion, and stimulated the secretion of a ~120-kDa gelatinase (metalloproteinase), but did not confer anchorage-independent growth or tumorigenicity in nude mice.
Treatment with a metalloproteinase inhibitor diminished ~120-kDa gelatinase activity in vitro and repressed the invasive phenotype of Id-1-expressing cells.
Expression of the ~120-kDa gelatinase naturally peaked during mammary gland involution, and elevated Id-1 along with gelatinase activity correlated with less-differentiated, aggressive human breast cancer phenotypes.

Cite This Study

Desprez et al. (1998) studied this question.

synapsesocial.com/papers/6a129ed41292a1e50c355035 https://doi.org/10.1128/mcb.18.8.4577

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Implication

In vitro and in vivo models show Id-1 induces metalloproteinase-driven cell invasion, suggesting a regulatory mechanism in mammary remodeling and aggressive breast cancer.

A Novel Pathway for Mammary Epithelial Cell Invasion Induced by the Helix-Loop-Helix Protein Id-1

Discussion

Member takes

Implication

Key Points

Cite This Study

A Novel Pathway for Mammary Epithelial Cell Invasion Induced by the Helix-Loop-Helix Protein Id-1

Discussion

Member takes

Implication

Key Points

Cite This Study

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