What question did this study set out to answer?

To identify discrete subpopulations of mature dendritic cells in mouse lymphoid tissues and determine whether their surface marker phenotypes remain stable after in vitro culture.

July 1, 1997The Journal of ImmunologyOpen Access

Dendritic cell subtypes in mouse lymphoid organs: cross-correlation of surface markers, changes with incubation, and differences among thymus, spleen, and lymph nodes

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To identify discrete subpopulations of mature dendritic cells in mouse lymphoid tissues and determine whether their surface marker phenotypes remain stable after in vitro culture.
Isolated mature dendritic cells from murine thymus, spleen, and lymph nodes.
Analyzed surface markers on freshly isolated and overnight-cultured cells using multicolor immunofluorescent labeling and flow cytometry.
Identified three primary dendritic cell subsets based on CD8alpha and DEC-205: thymus was dominated by CD8alpha+ DEC-205+ CD11b- cells, spleen contained equal proportions of CD8alpha+ DEC-205+ and CD8alpha- DEC-205- cells, and lymph nodes contained an additional CD8alpha- DEC-205+ CD11b- population.
Culturing cells overnight maintained distinct CD8alpha and DEC-205 expression levels between subsets, while CD11b, CD40, CD80, and L-selectin were uniformly up-regulated.

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Implication

Laboratory analysis reveals distinct dendritic cell subsets across mouse lymphoid organs, indicating lineage-specific functional diversity.

Cite This Study

Vremec et al. (1997) studied this question.

synapsesocial.com/papers/6a130616c031bb6829a7ba45 https://doi.org/10.4049/jimmunol.159.2.565

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Implication

Laboratory analysis reveals distinct dendritic cell subsets across mouse lymphoid organs, indicating lineage-specific functional diversity.

Dendritic cell subtypes in mouse lymphoid organs: cross-correlation of surface markers, changes with incubation, and differences among thymus, spleen, and lymph nodes

Key Points

Discussion

Implication

Cite This Study

Dendritic cell subtypes in mouse lymphoid organs: cross-correlation of surface markers, changes with incubation, and differences among thymus, spleen, and lymph nodes

Key Points

Discussion

Implication

Cite This Study

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