What question did this study set out to answer?

To examine whether the elastolytic matrix metalloproteinase 92-kD gelatinase is produced in abdominal aortic aneurysms and identify the cellular source responsible for its secretion.

July 1, 1995Journal of Clinical InvestigationOpen Access

Production and localization of 92-kilodalton gelatinase in abdominal aortic aneurysms. An elastolytic metalloproteinase expressed by aneurysm-infiltrating macrophages.

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Authors

RTRobert W. ThompsonHarvard University DHDennis R. HolmesBreast Cancer Research Foundation RMRenato MertensCleveland Clinic

Discussion

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Implication

Comparative tissue analysis reveals elevated macrophage 92-kilodalton gelatinase secretion in abdominal aortic aneurysm tissue, suggesting its role in chronic arterial wall degradation.

Key Points

To examine whether the elastolytic matrix metalloproteinase 92-kD gelatinase is produced in abdominal aortic aneurysms and identify the cellular source responsible for its secretion.
Cultured organ tissue from normal aorta, atheroocclusive disease (AOD), and abdominal aortic aneurysm (AAA) specimens with and without phorbol ester stimulation.
Quantified enzyme secretion using gelatin zymography and ELISA, and localized protein and mRNA expression via immunohistochemistry and in situ hybridization.
AAA tissues released approximately 2-fold more 92-kD gelatinase than AOD tissues and approximately 10-fold more than normal aorta, with phorbol ester inducing an 11.5-fold increase in AAA versus a 5.3-fold increase in normal and AOD tissues.
Immunohistochemistry and in situ hybridization localized 92-kD gelatinase protein and mRNA exclusively to infiltrating macrophages within the media and at the adventitial-medial junction across all AAA specimens.

Cite This Study

Thompson et al. (1995) studied this question.

synapsesocial.com/papers/6a14bfabbc94a5070a29db20 https://doi.org/10.1172/jci118037

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Implication

Comparative tissue analysis reveals elevated macrophage 92-kilodalton gelatinase secretion in abdominal aortic aneurysm tissue, suggesting its role in chronic arterial wall degradation.

Production and localization of 92-kilodalton gelatinase in abdominal aortic aneurysms. An elastolytic metalloproteinase expressed by aneurysm-infiltrating macrophages.

Discussion

Member takes

Implication

Key Points

Cite This Study

Production and localization of 92-kilodalton gelatinase in abdominal aortic aneurysms. An elastolytic metalloproteinase expressed by aneurysm-infiltrating macrophages.

Discussion

Member takes

Implication

Key Points

Cite This Study

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