The impact of quorum sensing and biofilm formation on antimicrobial resistance and virulence of XDR and MDR Pseudomonas aeruginosa in laying chickens.

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Background: plays a major influence on poultry outbreaks. Several factors may contribute to its pathogenicity. Aims: infection among layer chickens with phenotypic and genotypic characterization of the isolates. Methods: from Sharkia province, Egypt. The antimicrobial sensitivity was performed against 18 antimicrobial agents. A qualitative assessment of biofilm production was performed using the Tube method. The isolates were genetically examined for confirmation, detection of quorum sensing genes, virulence genes, and biofilm production genes by conventional PCR. Results: A were amplified in 100%, 84.2%, and 10.5%, respectively. Conclusion: A strong correlation between quorum sensing genes, biofilm genes, and virulence genes was detected. Further, biofilm production increases the resistance of the isolates to antimicrobial agents.