A polymerase chain reaction and hybridization assay successfully identified all reference strains and detected 28 of 29 clinical picornavirus isolates, discriminating enteroviruses from rhinoviruses.
PCR combined with hybridization using specific oligonucleotide probes is an effective method for the rapid detection and typing of human picornaviruses from cell culture.
We have used enzymic amplification of specific nucleic acid sequences followed by hybridization, for the rapid detection and typing of human picornaviruses after cell culture isolation. The test is based on the synthesis of cDNA, the polymerase chain reaction and the use of oligonucleotide probes. The primers were selected from the 5' non-coding region of the genome representing highly conserved regions. Sequences specific to enteroviruses and rhinoviruses were used as probes. The assay was able to identify all the picornavirus reference strains analysed and it was also possible to discriminate between enteroviruses and rhinoviruses by the hybridization procedure. When 29 picornavirus clinical isolates were analysed, all except one were detected by gel electrophoresis and a specific hybridization signal was obtained with all except three strains using the oligonucleotide probes.
Hyypiä et al. (1989) studied Human picornavirus infection (n=29). Polymerase chain reaction (PCR) and hybridization was evaluated on Detection and typing of human picornaviruses. A polymerase chain reaction and hybridization assay successfully identified all reference strains and detected 28 of 29 clinical picornavirus isolates, discriminating enteroviruses from rhinoviruses.
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