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January 1, 1993Journal of Clinical MicrobiologyOpen Access

PCR identified picornavirus in 146 (50%) of 292 symptomatic episodes compared to 47 (16%) by culture, representing a threefold greater detection rate.

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Why the study?

Does polymerase chain reaction improve the detection rate of picornavirus infection compared to viral culture in subjects with and without respiratory symptoms?

Population

108 children aged 9 to 11 years and 33 asymptomatic normal adults.

Comparison

Polymerase chain reaction amplification of the… vs Viral culture for three passages in Ohio HeLa…

Design

Cohort

Follow-up

1 year

Authors

SJSebastian L. JohnstonGSG SandersonPPPhilip Pattemore

Discussion

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Overview

PCR may improve picornavirus detection over culture in respiratory episodes; leaves open impact on management or outcomes.

Structured PICO

Does polymerase chain reaction improve the detection rate of picornavirus infection compared to viral culture in subjects with and without respiratory symptoms?

P
Population
108 children aged 9 to 11 years (evaluated during symptomatic episodes and when asymptomatic) and 33 asymptomatic normal adults.
I
Intervention
Polymerase chain reaction (PCR) amplification of the 5' noncoding region for identification of picornaviruses in nasal aspirates.
C
Comparator
Viral culture for three passages in Ohio HeLa cells at 33 degrees C and pH 7.0.
O
Outcome
Detection rate of picornavirus infection in nasal aspirates.surrogate

Polymerase chain reaction provides a threefold greater detection rate for picornavirus infection compared to traditional viral culture in both symptomatic and asymptomatic individuals.

Cite This Study

Johnston et al. (1993) studied this question.

synapsesocial.com/papers/6a100d4890ecb39bf65fc1f9https://doi.org/10.1128/jcm.31.1.111-117.1993
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Also Consider

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  1. 1Enzymatic RNA amplification of the enteroviruses1990 · 293 citations
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  3. 3Isolation of a monoclonal antibody that blocks attachment of the major group of human rhinoviruses1986 · 164 citations
  4. 4Polymerase Chain Reaction for Human Picornaviruses1989 · 227 citations
  5. 5Amplification of rhinovirus specific nucleic acids from clinical samples using the polymerase chain reaction1989 · 119 citations