Key result
The genetically encoded Ca2+ sensor R-CEPIA1er successfully measured Ca2+ uptake mediated by recombinant and native cardiac SERCA, providing a sensitive tool to study Ca2+ pump regulation.
Why the study?
SERCA2a plays a key role in cardiac Ca2+ regulation, but available techniques to study its function are either cell destructive or lack sensitivity.
Population
HEK293 cells expressing human SERCA2a and isolated mouse ventricular myocytes expressing R-CEPIA1er
Design
Preclinical experimental methodology and assay validation study
Authors
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May aid cellular SERCA research; leaves open translation to in vivo or clinical settings.
The genetically encoded Ca2+ sensor R-CEPIA1er provides a sensitive, non-destructive approach to selectively measure SERCA2a function and ER Ca2+ transport in cellular environments.
Bovo et al. (2019) studied Endoplasmic reticulum Ca2+ transport. R-CEPIA1er Ca2+ sensor was evaluated on SERCA2a function (ER Ca2+ uptake and leak). The genetically encoded Ca2+ sensor R-CEPIA1er successfully measured Ca2+ uptake mediated by recombinant and native cardiac SERCA, providing a sensitive tool to study Ca2+ pump regulation.
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