Key Points
- This research aims to characterize the polypeptides formed in response to EMC virus RNA in a cell-free system.
- Utilized cell-free systems from Krebs mouse ascites tumor cells to translate EMC virus RNA.
- Conducted electrophoresis on polyacrylamide gels and conducted fingerprint analysis of tryptic peptides.
- Characterized polypeptide synthesis over time to observe molecular weights and properties.
- Identified polypeptides ranged from 20,000 to 140,000 daltons, with some larger polypeptides seen.
- Major polypeptides arose from translating about 60% of the EMC RNA genome without cleavage of a precursor molecule.
- Indicated that products were likely artifacts due to premature termination during translation.
Structured PICO
PPopulationCell-free system from Krebs mouse ascites tumor cells
IInterventionAddition of encephalomyocarditis (EMC) virus ribonucleic acid (RNA)
OOutcomeCharacterization of polypeptide products synthesized at different times by electrophoresis on polyacrylamide gels and fingerprint analysis of their tryptic peptides
The study demonstrates that in a cell-free system from mouse ascites tumor cells, EMC virus RNA translation yields polypeptides that are likely artifacts of premature termination rather than cleavage of a large precursor.