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April 1, 1996The Journal of Cell BiologyOpen Access

Role of calmodulin and Spc110p interaction in the proper assembly of spindle pole body compenents.

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Authors

HSHolly SundbergMedizinische Hochschule HannoverLGLoretta GoetschUniversity of WashingtonBBBreck ByersUniversity of Washington

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Implication

Randomized trial shows the importance of calmodulin-Spc110p interaction for spindle assembly in yeast, highlighting its role in chromosome segregation.

Key Points

  • This research aims to explore the significance of the calmodulin-Spc110p interaction for the proper assembly of spindle pole body components in yeast.
  • Created temperature-sensitive mutations in SPC110 using PCR mutagenesis and plasmid shuffle strategy.
  • Utilized immunoelectron microscopy and immunofluorescent staining to analyze SPB components in mutant yeast cells.
  • Assessed the impact of calmodulin overproduction on temperature sensitivity of spc110-220 mutant.
  • Calmodulin overproduction suppresses the temperature sensitivity of the spc110-220 mutation.
  • Mutant cells showed significant lethality, with nuclear DNA segregation failure and spindle breakage.
  • Formation of an aberrant intranuclear microtubule organizer (IMO) observed in the absence of proper SPB assembly.

Cite This Study

Sundberg et al. (1996) studied this question.

synapsesocial.com/papers/6a15868ea2f71238514e819chttps://doi.org/10.1083/jcb.133.1.111
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1The essential mitotic target of calmodulin is the 110-kilodalton component of the spindle pole body in Saccharomyces cerevisiae.1993 · 146 citations
  2. 2Behavior of spindles and spindle plaques in the cell cycle and conjugation of Saccharomyces cerevisiae1975 · 616 citations
  3. 3Transcriptional role of yeast deoxyribonucleic acid dependent ribonucleic acid polymerase III1978 · 54 citations
  4. 4A rapid method for localized mutagenesis of yeast genes1992 · 456 citations
  5. 5Cytology of the Yeast Life Cycle1981 · 288 citations