What question did this study set out to answer?

Develop a selective, one-pot colorimetric assay using oligonucleotide-functionalized gold nanoparticle probes to detect and differentiate polynucleotide targets containing single-base mutations.

February 20, 1998Journal of the American Chemical Society

One-Pot Colorimetric Differentiation of Polynucleotides with Single Base Imperfections Using Gold Nanoparticle Probes

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Authors

JSJames J. StorhoffNanostring Technologies (United States)RERobert ElghanianNorthwestern University RMRobert C. MucicNorthwestern University

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Implication

In vitro experimental study demonstrates one-pot colorimetric differentiation of single-base polynucleotide imperfections using gold nanoparticle probes, suggesting a simple platform for genetic...

Key Points

Develop a selective, one-pot colorimetric assay using oligonucleotide-functionalized gold nanoparticle probes to detect and differentiate polynucleotide targets containing single-base mutations.
Synthesized ~13 nm gold nanoparticles capped with 3'- and 5'-(alkanethiol)oligonucleotides designed to hybridize to a 24-base target polynucleotide in a tail-to-tail orientation.
Tracked target-induced nanoparticle aggregation, surface plasmon resonance red shifts, and thermal melting transitions at 260 nm and 700 nm.
Spotted reaction mixtures onto C18 reverse-phase thin-layer chromatography plates to visually enhance color differentiation.
Target hybridization induced extended polymeric nanoparticle aggregation, triggering a distinct red-to-purple solution color change via surface plasmon resonance red-shifting.
Nanoparticle aggregates demonstrated sharp melting transitions capable of distinguishing fully complementary targets from sequences containing single-base end mismatches, deletions, or insertions.
Deposition onto C18 reverse-phase TLC plates produced an enhanced, permanent colorimetric readout enabling one-pot differentiation of target strands amidst single-base variant competitors.

Cite This Study

Storhoff et al. (1998) studied this question.

synapsesocial.com/papers/6a158e75a2352da347829ca2 https://doi.org/10.1021/ja972332i

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February 20, 1998Journal of the American Chemical Society

One-Pot Colorimetric Differentiation of Polynucleotides with Single Base Imperfections Using Gold Nanoparticle Probes

View Full Paper

Ask AI

Bookmark

Authors

JSJames J. StorhoffNanostring Technologies (United States)RERobert ElghanianNorthwestern University RMRobert C. MucicNorthwestern University

Discussion

Member takes

Implication

In vitro experimental study demonstrates one-pot colorimetric differentiation of single-base polynucleotide imperfections using gold nanoparticle probes, suggesting a simple platform for genetic...

Key Points

Develop a selective, one-pot colorimetric assay using oligonucleotide-functionalized gold nanoparticle probes to detect and differentiate polynucleotide targets containing single-base mutations.
Synthesized ~13 nm gold nanoparticles capped with 3'- and 5'-(alkanethiol)oligonucleotides designed to hybridize to a 24-base target polynucleotide in a tail-to-tail orientation.
Tracked target-induced nanoparticle aggregation, surface plasmon resonance red shifts, and thermal melting transitions at 260 nm and 700 nm.
Spotted reaction mixtures onto C18 reverse-phase thin-layer chromatography plates to visually enhance color differentiation.
Target hybridization induced extended polymeric nanoparticle aggregation, triggering a distinct red-to-purple solution color change via surface plasmon resonance red-shifting.
Nanoparticle aggregates demonstrated sharp melting transitions capable of distinguishing fully complementary targets from sequences containing single-base end mismatches, deletions, or insertions.
Deposition onto C18 reverse-phase TLC plates produced an enhanced, permanent colorimetric readout enabling one-pot differentiation of target strands amidst single-base variant competitors.

Cite This Study

Storhoff et al. (1998) studied this question.

synapsesocial.com/papers/6a158e75a2352da347829ca2 https://doi.org/10.1021/ja972332i

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