Rapid Naked‐Eye Discrimination of Cytochrome P450 Genetic Polymorphism through Non‐Crosslinking Aggregation of DNA‐Functionalized Gold Nanoparticles

This is the authors' abstract. We don't add key points for this paper.

(the variant allele with G681A point mutation). The present assay is composed of allele-specific single-base primer extension and salt-induced aggregation of DNA-modified gold nanoparticles (DNA-AuNPs). Genetic samples extracted from human hair roots are subjected to this assay. The results are verified by direct sequencing. This study should promise the prospective use of DNA-AuNPs in gene diagnosis.