Key result
Quantitative analysis of the phosphorylation status of multiple myofilament proteins can be successfully performed using 2-DE and Pro-Q Diamond stained gradient gels with minor amounts (~0.5 mg) of cardiac tissue.
This study provides a viable method for analyzing myofilament protein phosphorylation in very small cardiac biopsies, overcoming tissue availability limitations in human and animal studies.
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Allows myofilament phosphorylation analysis with minimal tissue; leaves open validation in human cardiac biopsies.
Zaremba et al. (2007) studied Cardiac tissue analysis. 2-DE and Pro-Q® Diamond stained gradient gels was evaluated on Quantitative analysis of phosphorylation status of multiple myofilament proteins. Quantitative analysis of the phosphorylation status of multiple myofilament proteins can be successfully performed using 2-DE and Pro-Q Diamond stained gradient gels with minor amounts (~0.5 mg) of cardiac tissue.
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