Quantitative analysis of the phosphorylation status of multiple myofilament proteins can be successfully performed using 2-DE and Pro-Q Diamond stained gradient gels with minor amounts (~0.5 mg) of cardiac tissue.
This study provides a viable method for analyzing myofilament protein phosphorylation in very small cardiac biopsies, overcoming tissue availability limitations in human and animal studies.
Phosphorylation of cardiac myofilament proteins represents one of the main post-translational mechanisms that regulate cardiac pump function. Human studies are often limited by the amount of available tissue as biopsies taken during cardiac catheterization weigh only 1 mg (dry weight). Similarly, investigation of time- (or dose-) dependent changes in protein phosphorylation in animal studies is often hampered by tissue availability. The present study describes quantitative analysis of phosphorylation status of multiple myofilament proteins by 2-DE and Pro-Q® Diamond stained gradient gels using minor amounts (˜0.5 mg dry weight) of human and pig cardiac tissue.
Zaremba et al. (Tue,) conducted a other in Cardiac tissue analysis. 2-DE and Pro-Q® Diamond stained gradient gels was evaluated on Quantitative analysis of phosphorylation status of multiple myofilament proteins. Quantitative analysis of the phosphorylation status of multiple myofilament proteins can be successfully performed using 2-DE and Pro-Q Diamond stained gradient gels with minor amounts (~0.5 mg) of cardiac tissue.