What is the clinical evidence from this study?

Study design: Other. Population: Canine ventricular muscle (basic science). Intervention: Purification of cardiac sarcolemmal vesicles. Primary outcome: Na+-dependent phosphoenzyme formation, ouabain binding, and ATP-dependent Ca2+ uptake.

What does this research mean for the field?

Cardiac sarcolemma contains an ATP-driven Ca2+ pump that is activated by calmodulin and operates independently of Na+ and H+ transmembrane gradients. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

The aim is to purify cardiac sarcolemmal vesicles and evaluate their sodium pump content and calcium uptake mechanisms.

April 1, 1982The Journal of Biochemistry

Purification of Cardiac Sarcolemmal Vesicles: High Sodium Pump Content and ATP-Dependent, Calmodulin-Activated Calcium Uptake1

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Key result

Highly purified cardiac sarcolemmal vesicles from canine ventricular muscle demonstrated an ATP-driven Ca2+ pump that is activated by calmodulin and independent of Na+ and H+ gradients.

Population

Highly purified vesicles of cardiac sarcolemma prepared from a homogenate of canine ventricular muscle

Design

Preclinical

Authors

HKHideto KuwayamaObihiro University of Agriculture and Veterinary Medicine TKTohru KanazawaTokyo Institute of Technology

Discussion

Member takes

Implication

Provides evidence for calmodulin-activated sarcolemmal Ca2+ pump in canine heart; hypothesis-generating for human cardiomyocyte calcium regulation.

Key Points

The aim is to purify cardiac sarcolemmal vesicles and evaluate their sodium pump content and calcium uptake mechanisms.
Used density gradient centrifugation to prepare highly purified cardiac sarcolemma vesicles from canine ventricular muscle.
Assessed (Na+,K+)-ATPase content and calcium uptake under various conditions with inhibitors and activators.
Measured phosphoenzyme formation levels with and without Triton X-100.
Demonstrated high (Na+,K+)-ATPase content with levels of 773 and 907 pmol.mg-1 under optimal conditions.
Showed ATP-dependent calcium uptake, with rapid release upon sodium chloride addition, indicating the presence of a calcium pump.
Half-maximum activation of the calcium pump by calmodulin was observed at a concentration of 0.05 micrograms.ml-1.

Structured PICO

Population

Highly purified vesicles of cardiac sarcolemma prepared from a homogenate of canine ventricular muscle

Intervention

ATP, calmodulin, and various inhibitors (digitoxin, monensin, dinitrophenol, trifluoperazine)

Outcome

ATP-dependent Ca2+ uptake and phosphoenzyme formationsurrogate

Demonstrates the existence of an ATP-driven, calmodulin-activated calcium pump in the cardiac sarcolemma using highly purified canine ventricular vesicles.

Cite This Study

Kuwayama et al. (1982) studied Canine ventricular muscle (basic science). Purification of cardiac sarcolemmal vesicles was evaluated on Na+-dependent phosphoenzyme formation, ouabain binding, and ATP-dependent Ca2+ uptake. Highly purified cardiac sarcolemmal vesicles from canine ventricular muscle demonstrated an ATP-driven Ca2+ pump that is activated by calmodulin and independent of Na+ and H+ gradients.

synapsesocial.com/papers/6a1598a35347fbb173a00517 https://doi.org/10.1093/oxfordjournals.jbchem.a133830

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Implication

Provides evidence for calmodulin-activated sarcolemmal Ca2+ pump in canine heart; hypothesis-generating for human cardiomyocyte calcium regulation.

Purification of Cardiac Sarcolemmal Vesicles: High Sodium Pump Content and ATP-Dependent, Calmodulin-Activated Calcium Uptake1

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Purification of Cardiac Sarcolemmal Vesicles: High Sodium Pump Content and ATP-Dependent, Calmodulin-Activated Calcium Uptake1

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

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