Key result
Low-cost open-source optical mapping provides ~16 SNR for accurate action potential measurements in rodent hearts.
Why the study?
High-speed CMOS cameras capable of optical registration of cardiac action potential propagation are generally very costly.
A low-cost (<$1,000) open-source optical mapping system provides adequate signal quality for cardiac electrophysiology and drug studies in rodent models.
Supports low-cost optical mapping in rodent models; leaves open validation in larger species or clinical settings.
Fluorescent imaging with voltage- or calcium-sensitive dyes, i . e . optical mapping, is one of the indispensable modern techniques to study cardiac electrophysiology, unsurpassed by temporal and spatial resolution. High-speed CMOS cameras capable of optical registration of action potential propagation are in general very costly. We present a complete solution priced below US$ 1,000 (including camera and lens) at the moment of publication with an open-source image acquisition and processing software. We demonstrate that the iDS UI-3130CP rev.2 camera we used in this study is capable of 200×200 977 frames per second (FPS) action potential recordings from rodent hearts. The signal-to-noise-ratio of a conditioned signal was 16 ± 10 for rodent hearts. A comparison with a specialized MiCAM Ultimate-L camera has shown that signal-to-noise ratio (SNR) is sufficient for accurate measurements of AP waveform, conduction velocity (± 0.04 m/s) and action potential duration (± 7ms) in mouse and rat hearts. We measured the action potential prolongation during 4-aminopyridine administration in mouse heart, showing that proposed system signal quality is adequate for drug studies.
No takes yet. Share an insight, caveat, or question.
Rybashlykov et al. (2021) studied Cardiac electrophysiology. Open-source low-cost cardiac optical mapping system (iDS UI-3130CP rev.2 camera) vs. Specialized MiCAM Ultimate-L camera was evaluated on Signal-to-noise ratio, action potential waveform, conduction velocity, and action potential duration. An open-source, low-cost cardiac optical mapping system provided sufficient signal-to-noise ratio (16 ± 10) for accurate measurements of action potential waveform and conduction velocity in rodent hearts.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: