A DNAzyme with Three Protein‐Like Functional Groups: Enhancing Catalytic Efficiency of M ²⁺ ‐Independent RNA Cleavage

Silence of the small motif: Dz10-66 functions at low monovalent ionic strength and exhibits the highest M2+-independent self-cleavage rate reported to date for any nucleic acid catalyst. Dz10-66 also exhibits a catalytic efficiency similar to that of other known Mg2+-dependent DNAzymes. The “tyranny of the small motif”, whereby certain subsequences dominate selections of increased library size did not prevail in the selection of a new DNAzyme with three protein-like functionalities that catalyses intra- and intermolecular RNA cleavage. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.