What question did this study set out to answer?

To identify methods that reduce antibody-mediated sample interference in an in-house two-site, two-step immunometric assay.

April 1, 2002Clinical ChemistryOpen Access

Immunometric Assay Interference

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Authors

JBJohan Bjerner181st Hospital of Chinese People's Liberation Army KNKjell NustadOslo University Hospital LNLars F. NorumUniversity of Oslo

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Implication

Comparative laboratory study reveals that heat-treated murine immunoglobulin and Fc fragment removal reduce interference in serum assays, indicating practical solutions for diagnostic accuracy.

Key Points

To identify methods that reduce antibody-mediated sample interference in an in-house two-site, two-step immunometric assay.
Screened 11,261 routine patient serum samples using a carcinoembryonic antigen (CEA) assay lacking murine immunoglobulins in the buffer alongside modified assay protocols.
Evaluated the effect of adding native nonspecific murine immunoglobulin (MAK33 at 15 mg/L), heat-treated MAK33 (15 and 50 mg/L), and enzymatic removal of Fc fragments from the capture antibody.
Compared demographic characteristics between interference-positive cases (n = 210) and negative controls (n = 186), and tracked interference over time across serial samples from 25 patients.
Baseline interference frequency was 4.0% (95% CI, 3.3–4.7%); native MAK33 had minimal effect (3.9%; 95% CI, 3.2–4.6%), while heat-treated MAK33 reduced interference to 0.86% at 15 mg/L (95% CI, 0.61–1.12%) and 0.06% at 50 mg/L (95% CI, 0–0.13%).
Removal of Fc fragments from the capture antibody alone lowered the interference frequency to 0.10% (95% CI, 0.02–0.19%).
Interference did not differ by age (P < 0.23) or sex (P < 0.40), and 15 of 25 evaluated individuals exhibited transient interference with negative results before or after the interference peak.

Cite This Study

Bjerner et al. (2002) studied this question.

synapsesocial.com/papers/6a15c6cea2f71238514eb65d https://doi.org/10.1093/clinchem/48.4.613

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Implication

Comparative laboratory study reveals that heat-treated murine immunoglobulin and Fc fragment removal reduce interference in serum assays, indicating practical solutions for diagnostic accuracy.

Immunometric Assay Interference

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Member takes

Implication

Key Points

Cite This Study

Immunometric Assay Interference

Discussion

Member takes

Implication

Key Points

Cite This Study

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