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This document is a media kit for Cold Spring Harbor Laboratory Press journals and does not contain clinical trial data.
Provides a modified protocol for determining the apparent Ca2+ affinity of the Ca2+ pump in crude homogenates of mouse heart.
No clinical data here; leaves open translation of this mouse heart Ca2+ pump protocol to human studies.
The apparent Ca(2+) affinity of the isoforms of the sarco/endoplasmic reticulum Ca(2+) ATPase SERCA2 is controlled primarily by two proteins, phospholamban (PLB) and sarcolipin (SLN). The rate of ATP-driven Ca(2+) uptake into sarcoplasmic reticulum (SR)-derived vesicles can be monitored by a technique in which the net uptake of (45)Ca(2+) in the form of an intravesicular calcium oxalate precipitate is recorded. Here, we present details of a modification of such a protocol for determining the apparent Ca(2+) affinity of the Ca(2+) pump, and its control by various regulators, in crude homogenates of mouse heart.
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Holemans et al. (2014) studied this question. This document is a media kit for Cold Spring Harbor Laboratory Press journals and does not contain clinical trial data.
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