What does this research mean for the field?

A reduction in SERCA2 protein levels directly impairs intracellular Ca2+ homeostasis and cardiomyocyte contractility, but these deficits are partially mitigated by compensatory decreases in phospholamban inhibition and up-regulation of the Na+-Ca2+ exchanger. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

December 1, 2000ViewOpen Access

Disruption of a Single Copy of the SERCA2 Gene Results in Altered Ca2+ Homeostasis and Cardiomyocyte Function

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Population

Isolated cardiomyocytes from a mouse model carrying a null mutation in one copy of the SERCA2 gene

Comparison

Null mutation in one copy of the SERCA2 gene… vs Wild-type controls

Design

Preclinical

Authors

YJYong JiNingbo University MLM. Jane LalliThe Ohio State University GBGopal J. BabuRutgers, The State University of New Jersey

Discussion

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Implication

Reduced SERCA2 impairs myocyte Ca2+ handling and contractility in mice; leaves open relevance to human HF.

Structured PICO

Population

Isolated cardiomyocytes from a mouse model carrying a null mutation in one copy of the SERCA2 gene (SERCA2 heterozygous mice)

Intervention

Null mutation in one copy of the SERCA2 gene resulting in approximately 35% reduction in SERCA2 protein levels

Comparator

Wild-type controls

Outcome

Intracellular Ca(2+) homeostasis and contractile properties (SR Ca(2+) stores, Ca(2+) release, rate of myocyte shortening and relengthening)surrogate

Reduced SERCA2 levels directly impair intracellular Ca2+ homeostasis and myocyte contractility, though partial compensation occurs through altered phospholamban interactions and Na+-Ca2+ exchanger upregulation.

Cite This Study

Ji et al. (2000) studied this question.

synapsesocial.com/papers/6a1beef41567d2fc4d5f47bf https://doi.org/10.1074/jbc.m004804200

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Disruption of a Single Copy of the SERCA2 Gene Results in Altered Ca2+ Homeostasis and Cardiomyocyte Function

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Disruption of a Single Copy of the SERCA2 Gene Results in Altered Ca2+ Homeostasis and Cardiomyocyte Function

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider