What question did this study set out to answer?

To investigate the signaling mechanisms and regulatory cross-talk between light perception and ABA-independent cold-stress response pathways in Arabidopsis thaliana.

March 1, 2002The Plant JournalOpen Access

Light signalling mediated by phytochrome plays an important role in cold‐induced gene expression through the C‐repeat/dehydration responsive element (C/DRE) in Arabidopsis thaliana

View Full Paper

Ask AI

Bookmark

Authors

HKHyoun‐Joung KimNongwoo Bio (South Korea)YKYun‐Kyoung KimSeoul National University JPJin‐Young ParkSeoul National University

Discussion

Member takes

Implication

Laboratory study reveals phytochrome B activates cold-responsive gene expression in Arabidopsis thaliana, indicating light signaling directly enhances freezing tolerance pathways.

Key Points

To investigate the signaling mechanisms and regulatory cross-talk between light perception and ABA-independent cold-stress response pathways in Arabidopsis thaliana.
Generated transgenic Arabidopsis thaliana expressing a GUS reporter gene driven by four copies of the cor15a C/DRE motif with a minimal promoter (4C/DRE-GUS).
Subjected plants to cold, drought, red light, far-red light, and 1 nM okadaic acid to measure reporter expression.
Evaluated cold-induced GUS and endogenous CBF1, 2, 3, and cor15a expression kinetics in wild-type, phyA, phyB, and phyAphyB mutant backgrounds.
Cold-induced reporter expression was inhibited by 1 nM okadaic acid, identifying protein phosphatase 2A as a putative positive regulator.
Red/far-red photoreversibility and mutant assays established phytochrome B as the primary photoreceptor mediating light-activated cold-stress signaling.
Light accelerated induction kinetics of CBF1, CBF2, CBF3, and downstream cor15a relative to darkness, situating phytochrome-mediated signaling upstream of CBF transcription.

Cite This Study

Kim et al. (2002) studied this question.

synapsesocial.com/papers/6a15d2a2c9af5fd377521cfa https://doi.org/10.1046/j.1365-313x.2002.01249.x

View Full Paper

Ask AI

Bookmark

Implication

Laboratory study reveals phytochrome B activates cold-responsive gene expression in Arabidopsis thaliana, indicating light signaling directly enhances freezing tolerance pathways.

Light signalling mediated by phytochrome plays an important role in cold‐induced gene expression through the C‐repeat/dehydration responsive element (C/DRE) in Arabidopsis thaliana

Discussion

Member takes

Implication

Key Points

Cite This Study

Light signalling mediated by phytochrome plays an important role in cold‐induced gene expression through the C‐repeat/dehydration responsive element (C/DRE) in Arabidopsis thaliana

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider