Key result
Endotoxin-induced systemic inflammation in healthy volunteers increased VWF levels by 85% and decreased collagen epinephrine and ADP closure times by 47% and 38% respectively.
Why the study?
Does endotoxin-induced systemic inflammation alter platelet plug formation measured by PFA-100 in healthy volunteers?
RCT
Randomized
Does endotoxin-induced systemic inflammation alter platelet plug formation measured by PFA-100 in healthy volunteers?
Acute systemic inflammation significantly alters PFA-100 platelet function test results, which may confound clinical interpretation.
Answers are grounded in this paper and cite their sources.
May confound PFA-100 interpretation during acute inflammation; extends causal RCT evidence on inflammation effects in volunteers.
The PFA-100 measures platelet plug formation under shear stress and is strongly dependent on von Willebrand Factor (VWF) levels in plasma. We therefore hypothesized that elevated VWF levels, possibly as a result of acute inflammation, adversely influence PFA-100 results. Healthy volunteers received either 2 ng/kg endotoxin or placebo in a randomized controlled trial. Four hours after endotoxin (but not placebo) infusion VWF levels increased by 85%, collagen epinephrine-induced closure time (CT) decreased by 47% and collagen ADP-CT decreased by 38% (P < 0.0001) respectively. In conclusion, systemic inflammation has a major impact on the results obtained by PFA-100 and may confound interpretation of platelet function.
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Homoncik et al. (2000) conducted an RCT in Healthy volunteers. Endotoxin vs. Placebo was evaluated on VWF levels and PFA-100 closure times (collagen epinephrine-induced CT and collagen ADP-CT) (p=< 0.0001). Endotoxin-induced systemic inflammation in healthy volunteers increased VWF levels by 85% and decreased collagen epinephrine and ADP closure times by 47% and 38% respectively.
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