Key result
Interaction with magnesium dication causes variations in proton chemical shifts and splitting of the H8 resonance peak of G-actin-bound ATP adenine, indicating hydrolysis to ADP-G-actin.
The study provides NMR-based evidence that magnesium dication induces the hydrolysis of monomeric ATP-G-actin to ADP-G-actin, marking the initial stage of F-actin formation.
NMR proton spectra were recorded in the range of proton resonance in the nucleotide aromatic ring of monomeric ATP–G-actin and the Mg2+–ATP–G-actin solutions in D2O to study the mechanism of ATP–G-actin hydrolysis and its role in F-actin formation in Mg2+-containing solutions. The experimental data show variations in the proton chemical shifts of the H2 and H8 peaks and splitting of the H8 resonance peak of G-actin-bound ATP adenine caused by interaction with magnesium dication. The observed variations in spectra are explained by hydrolysis of monomeric ATP–G-actin to ADP–G-actin, which is regarded as the initial stage of the G-actin to F-actin transformation.
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V. N. Umetskaya (2016) studied this question. Mg2+-containing solutions vs. monomeric ATP-G-actin without Mg2+ was evaluated on Proton chemical shifts of H2 and H8 peaks and splitting of H8 resonance peak. Interaction with magnesium dication causes variations in proton chemical shifts and splitting of the H8 resonance peak of G-actin-bound ATP adenine, indicating hydrolysis to ADP-G-actin.
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