Key result
Sequencing of MAb neutralization-resistant mutants of a European strain of swine vesicular disease virus identified four surface-exposed neutralizing epitopes, including one novel to picornaviruses.
Identified four neutralizing epitopes on a European strain of swine vesicular disease virus, including a novel epitope in the C-terminal region of VP3 not previously recognized in picornaviruses.
Extends SVDV epitope mapping beyond Japanese strains; leaves open implications for picornavirus antigen design.
Six neutralizing monoclonal antibodies (MAbs) were used to isolate MAb neutralization-resistant (MAR) mutants from a recent European strain of swine vesicular disease virus (SVDV), ITL/9/93. Sequencing of MAR mutants identified two epitopes located at positions analogous to sites 2A (VP2) and 3B (VP3) on poliovirus (PV) which have been previously identified on a Japanese strain of SVDV. A third epitope near to the C terminus of VP1, not previously recognized on SVDV, was tentatively identified in a region analogous to site 1 of PV. A fourth epitope, located in the C-terminal region of VP3, has never before been recognized as a site of neutralization on picornaviruses. All four epitopes were predicted to be surface-exposed.
No takes yet. Share an insight, caveat, or question.
Nijhar et al. (1999) studied Swine vesicular disease virus (SVDV). Monoclonal antibodies (MAbs) was evaluated on Identification of neutralizing epitopes. Sequencing of MAb neutralization-resistant mutants of a European strain of swine vesicular disease virus identified four surface-exposed neutralizing epitopes, including one novel to picornaviruses.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: