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September 1, 1982Journal of VirologyOpen Access

Poliovirus neutralization epitopes: analysis and localization with neutralizing monoclonal antibodies

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Population

Poliovirus type 1 (Mahoney strain) and hybridomas (H3 and D3) secreting monoclonal neutralizing antibodies

Design

Preclinical

Authors

EEEmilio A. EminiUniversity of North Carolina at Chapel HillBJBradford A. JamesonAllegheny CollegeALAngela LewisSouth London and Maudsley NHS Foundation Trust

Discussion

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Implication

Should not yet inform poliovirus vaccine strategies; leaves open VP1 epitope conservation and immunogenicity in humans.

Key Points

  • To characterize and localize distinct neutralization epitopes on intact type 1 poliovirus particles using monoclonal neutralizing antibodies.
  • Generated hybridomas H3 and D3 producing monoclonal neutralizing antibodies against intact poliovirus type 1 (Mahoney strain).
  • Tested antibody reactivity across intact virions, 80S empty capsids, 14S precursor subunits, heat-denatured virions, and isolated viral proteins.
  • Identified antibody binding sites on capsid proteins via covalent cross-linking of antibody F(ab) fragments using toluene-2,4-diisocyanate.
  • Antibody H3 recognized intact virions, 80S empty capsids, and 14S precursors, while antibody D3 bound only intact virions and empty capsids; neither antibody bound heat-treated virions or denatured proteins.
  • Neutralization-resistant viral escape variants arose spontaneously during cell culture passage at a frequency of approximately 10^-4 per wild-type plaque-forming unit.
  • Covalent cross-linking identified capsid structural protein VP1 as the carrier of both distinct neutralization epitopes.

Structured PICO

P
Population
Poliovirus type 1 (Mahoney strain) and hybridomas (H3 and D3) secreting monoclonal neutralizing antibodies
I
Intervention
Monoclonal neutralizing antibodies (H3 and D3) and cross-linking with Toluene-2,4-diisocyanate
O
Outcome
Identification of neutralization epitopes and structural protein containing binding sitessurrogate

VP1 is the structural protein containing the neutralization epitopes for monoclonal antibodies H3 and D3 in poliovirus type 1.

Cite This Study

Emini et al. (1982) studied this question.

synapsesocial.com/papers/6a7eae3ababc2b64f88b9b97https://doi.org/10.1128/jvi.43.3.997-1005.1982
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Also Consider

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  1. 1Immune and Antibody Responses to an Isolated Capsid Protein of Foot-and-Mouth Disease Virus1975 · 216 citations
  2. 2Multiple Genetic Changes Can Occur in the Oral Poliovaccines upon Replication in Humans1981 · 148 citations
  3. 3Principles of Immunization as Applied to Poliomyelitis and Influenza1953 · 26 citations
  4. 4Identification of the initiation site of poliovirus polyprotein synthesis1982 · 89 citations