What question did this study set out to answer?

To elucidate the functional mechanisms and epitope specificity mediating antibody-directed neutralization of poliovirus.

May 1, 1983Journal of VirologyOpen Access

Functional basis of poliovirus neutralization determined with monospecific neutralizing antibodies

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Population

Poliovirus and 13 monospecific neutralizing antibodies

Design

Preclinical

Authors

EEEmilio A. EminiUniversity of North Carolina at Chapel Hill SKS Y KaoUniversity of Colorado Denver ALAngela LewisSouth London and Maudsley NHS Foundation Trust

Discussion

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Implication

No immediate change to poliovirus vaccination; leaves open whether pI shifts inform epitope-targeted human vaccines.

Key Points

To elucidate the functional mechanisms and epitope specificity mediating antibody-directed neutralization of poliovirus.
Characterized 13 monospecific neutralizing antibodies targeting seven distinct viral epitopes on poliovirus virions.
Assayed antibody effects on cellular attachment, viral penetration, virus-specific transcription, and alterations in virion isoelectric point (pI).
Epitope-specific viral variation frequencies ranged from -3.1 to -4.2 log10 variant PFU per wild-type PFU across the targeted epitopes.
Neutralizing antibodies did not fully block cellular attachment or viral penetration under nonsaturating conditions, but all tested antibodies completely prevented virus-specific transcription.
Antibody binding strongly correlated with a major shift in the virion's isoelectric point from pI 7.0 to approximately 4.0 for all but one epitope.

Structured PICO

Population

Poliovirus and 13 monospecific neutralizing antibodies

Intervention

13 monospecific neutralizing antibodies

Outcome

Antibody-mediated poliovirus neutralization mechanisms (binding, penetration, transcription, pI shift)

Poliovirus neutralization by antibodies primarily occurs by preventing virus-specific transcription and altering the virion's isoelectric point, rather than blocking cell binding or penetration.

Cite This Study

Emini et al. (1983) studied this question.

synapsesocial.com/papers/6a7d604aa3b6fd5035d5b3a8 https://doi.org/10.1128/jvi.46.2.466-474.1983

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Functional basis of poliovirus neutralization determined with monospecific neutralizing antibodies

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Functional basis of poliovirus neutralization determined with monospecific neutralizing antibodies

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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