ABSTRACT Zinc oxide nanoparticles (ZnO NPs) are promising bioactive agents for wound management due to their antimicrobial and regenerative properties, and their ability to induce analgesia in animal models. Vitamins A and E are known to support tissue repair and modulate inflammatory pain. This study evaluates a topical ointment combining green‐synthesized ZnO NPs (derived from Prosopis juliflora leaf extract) with vitamins A and E, for wound‐healing, analgesic, and antibacterial efficacy in a rat excisional wound model. Four ointment formulations were applied to adult male Wistar rats for 14 days. ZnO NP‐containing formulations exhibited clear antibacterial effects, with the vitamin A + ZnO NP combination showing the strongest activity against Escherichia coli , Pseudomonas aeruginosa , and Staphylococcus aureus . In the wound model, the combined vitamin/ZnO NP ointment significantly accelerated wound closure, reduced wound area, improved epithelial thickness and collagen organization, and produced the strongest analgesic effect among the tested formulations. This treatment also markedly increased VEGF and TGF‐β expression, indicating enhanced tissue regeneration. The analgesic effect may be associated, at least in part, with modulation of NMDA‐related pain pathways. These findings suggest a beneficial interaction between green‐synthesized ZnO NPs and vitamins A/E, supporting their potential in multifunctional topical wound‐care formulations.
Torabi et al. (Fri,) studied this question.