What is the clinical evidence from this study?

Population: Hypertension caused by angiotensin II. Intervention: Angiotensin II infusion vs. Wild-type (WT) mice. Primary outcome: Systolic blood pressure (mm Hg) (p=<0.05).

What does this research mean for the field?

The NAD(P)H oxidase subunit p47(phox) is essential for angiotensin II-induced vascular oxidative stress and hypertension in vivo, as its deficiency markedly blunts the hypertensive response and prevents increases in vascular superoxide. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

This study aims to determine the role of the p47phox subunit in vascular oxidative stress and hypertension induced by angiotensin II.

October 1, 2002HypertensionOpen Access

Role of p47 phox in Vascular Oxidative Stress and Hypertension Caused by Angiotensin II

Q: What are the key findings of this study?

In p47(phox-/-) mice, the hypertensive response to angiotensin II infusion was markedly blunted compared to wild-type mice (122+/-4 vs 151+/-6 mm Hg; P<0.05), with no increase in vascular superoxide.

Q: What does this research mean for the field?

The NAD(P)H oxidase subunit p47(phox) is essential for angiotensin II-induced vascular oxidative stress and hypertension in vivo, as its deficiency markedly blunts the hypertensive response and prevents increases in vascular superoxide. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

Q: What question did this study set out to answer?

This study aims to determine the role of the p47phox subunit in vascular oxidative stress and hypertension induced by angiotensin II.

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Key result

In p47(phox-/-) mice, the hypertensive response to angiotensin II infusion was markedly blunted compared to wild-type mice (122+/-4 vs 151+/-6 mm Hg; P<0.05), with no increase in vascular superoxide.

Key Points

This study aims to determine the role of the p47phox subunit in vascular oxidative stress and hypertension induced by angiotensin II.
Used p47phox-deficient mice to assess the effects of angiotensin II on blood pressure and oxidative stress.
Infused angiotensin II at 0.7 mg/kg per day for 7 days to measure systolic blood pressure and vascular O2*- production.
Conducted in situ staining and Western blotting to analyze the presence and activity of O2*- in vascular tissues.
Angiotensin II infusion raised systolic blood pressure significantly in WT mice from 105±2 to 151±6 mm Hg.
In p47phox-/- mice, blood pressure only increased to 122±4 mm Hg (P<0.05) with no O2*- elevation.
Dihydroethidium staining demonstrated O2*- production in WT mice but not in p47phox-/- mice.

Structured PICO

Population

Wild-type (WT) mice and p47(phox-/-) mice (deficient in p47phox, a cytosolic subunit of NADPH oxidase), as well as cultured endothelial cells from these mice.

Intervention

Angiotensin II infusion (0.7 mg/kg per day for 7 days)

Comparator

Wild-type mice compared to p47(phox-/-) mice, both receiving angiotensin II infusion

Outcome

Systolic blood pressure and vascular superoxide (O2*-) formationsurrogate

Main Result

Absolute Event Rate: 122% vs 151%

p-value: p=<0.05

The NAD(P)H oxidase subunit p47phox plays a pivotal role in mediating vascular oxidative stress and the hypertensive response to angiotensin II in vivo.

Discussion

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Implication

p47phox deletion attenuates Ang II hypertension in mice; extends in vitro data but leaves open human therapeutic targeting.

Cite This Study

Landmesser et al. (2002) studied Hypertension caused by angiotensin II. Angiotensin II infusion vs. Wild-type (WT) mice was evaluated on Systolic blood pressure (mm Hg) (p=<0.05). In p47(phox-/-) mice, the hypertensive response to angiotensin II infusion was markedly blunted compared to wild-type mice (122+/-4 vs 151+/-6 mm Hg; P<0.05), with no increase in vascular superoxide.

synapsesocial.com/papers/6a169ecc83b2be9fec6b45b9 https://doi.org/10.1161/01.hyp.0000032100.23772.98

Topics

Hypertension management

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Role of p47 phox in Vascular Oxidative Stress and Hypertension Caused by Angiotensin II

Key Points

Structured PICO

Main Result

Discussion

Implication

Cite This Study

Topics

Role of p47 phox in Vascular Oxidative Stress and Hypertension Caused by Angiotensin II

Key Points

Structured PICO

Main Result

Discussion

Implication

Cite This Study

Also Consider

Topics

Also Consider