What question did this study set out to answer?

To determine whether interleukin-6 functions as an autocrine growth factor supporting the proliferation of human renal cell carcinoma cells.

July 3, 1989FEBS LettersOpen Access

Interleukin‐6 (IL‐6) functions as an in vitro autocrine growth factor in renal cell carcinomas

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In vitro study demonstrates that interleukin-6 promotes tumor proliferation in human renal cell carcinoma, indicating an autocrine growth mechanism.

To determine whether interleukin-6 functions as an autocrine growth factor supporting the proliferation of human renal cell carcinoma cells.
Cultured freshly isolated patient-derived renal cell carcinoma cells with and without fetal calf serum.
Measured interleukin-6 mRNA expression, protein secretion, and intracellular localization via antiserum immunostaining.
Tested the effect of anti-interleukin-6 antiserum on in vitro tumor cell proliferation.
Freshly isolated renal cell carcinoma cells expressed interleukin-6 mRNA and secreted active interleukin-6 in serum-supported culture, but failed to produce interleukin-6 or proliferate in serum-free conditions.
Immunostaining with anti-interleukin-6 antiserum verified endogenous interleukin-6 production within the carcinoma cells.
Addition of anti-interleukin-6 antiserum specifically inhibited in vitro tumor cell growth.

Miki et al. (1989) studied this question.

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In vitro study demonstrates that interleukin-6 promotes tumor proliferation in human renal cell carcinoma, indicating an autocrine growth mechanism.