What question did this study set out to answer?

To identify and characterize surface membrane glycoprotein defects in platelets isolated from patients with Glanzmann's thrombasthenia.

September 1, 1977Journal of Clinical InvestigationOpen Access

Platelet Membrane Defects in Glanzmann's Thrombasthenia

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Authors

DPDavid R. PhillipsBrigham Young University PAPatricia Poh AginBH Consulting (Ireland)

Discussion

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Implication

Laboratory analysis demonstrates marked deficiencies of membrane glycoproteins IIb and III in Glanzmann's thrombasthenia, indicating structural surface defects underlying platelet dysfunction.

Key Points

To identify and characterize surface membrane glycoprotein defects in platelets isolated from patients with Glanzmann's thrombasthenia.
Labeled membrane surface polypeptides of normal and thrombasthenic platelets using lactoperoxidase-catalyzed iodination and galactose oxidase oxidation with tritiated sodium borohydride.
Separated labeled membrane proteins by two-dimensional polyacrylamide gel electrophoresis under reducing conditions using beta-mercaptoethanol.
Assessed glycoprotein expression levels via Coomassie Blue and periodic acid-Schiff staining, and examined carbohydrate binding using Lens culinaris lectin affinity chromatography.
Thrombasthenic platelets exhibited radioactive labeling reductions of approximately 60% for glycoprotein IIb and 80% for glycoprotein III relative to normal controls.
Reduced staining with Coomassie Blue and periodic acid-Schiff reagent confirmed that the labeling deficit was driven by decreased glycoprotein concentration rather than steric inaccessibility.
Affinity chromatography revealed that glycoprotein IIb possesses receptors for Lens culinaris lectin, whereas glycoprotein III does not.

Cite This Study

Phillips et al. (1977) studied this question.

synapsesocial.com/papers/6a16f4b187c007abbc05412d https://doi.org/10.1172/jci108805

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Implication

Laboratory analysis demonstrates marked deficiencies of membrane glycoproteins IIb and III in Glanzmann's thrombasthenia, indicating structural surface defects underlying platelet dysfunction.

Platelet Membrane Defects in Glanzmann's Thrombasthenia

Discussion

Member takes

Implication

Key Points

Cite This Study

Platelet Membrane Defects in Glanzmann's Thrombasthenia

Discussion

Member takes

Implication

Key Points

Cite This Study

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