What is the clinical evidence from this study?

Study design: Other. Population: Vascular smooth muscle contraction. Intervention: Phenylephrine stimulation. Primary outcome: Smooth muscle cell contraction and protein interactions.

What does this research mean for the field?

Phenylephrine induces contraction of ferret aorta cells without increasing intracellular calcium or myosin light chain phosphorylation, accompanied by MAPK activation and calponin redistribution to the cell membrane. Novelty: ClaimNovelty.CONTRADICTORY. Consensus alignment: ConsensusAlignment.CHALLENGES_CONSENSUS.

What question did this study set out to answer?

To investigate the mechanisms underlying calcium-independent vascular smooth muscle contraction and determine the signaling role of calponin with mitogen-activated protein kinase (MAPK).

October 1, 1997Journal of Biological ChemistryOpen Access

Calponin and Mitogen-activated Protein Kinase Signaling in Differentiated Vascular Smooth Muscle

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Key result

Phenylephrine induces contraction of ferret aorta cells without increasing intracellular calcium, accompanied by MAPK activation and calponin redistribution to the cell membrane.

Population

Freshly isolated ferret aorta cells

Design

Preclinical

Authors

CMConstance B. MeniceBoston Biomedical Research Institute JHJustin HulvershornBoston Biomedical Research Institute LALeonard P. AdamBristol-Myers Squibb (United States)

Discussion

Member takes

Implication

Challenges Ca2+-dependent contraction paradigm in vascular smooth muscle; hypothesis-generating in animal models, requiring human validation.

Key Points

To investigate the mechanisms underlying calcium-independent vascular smooth muscle contraction and determine the signaling role of calponin with mitogen-activated protein kinase (MAPK).
Stimulated freshly isolated ferret aortic smooth muscle cells with phenylephrine under calcium-free extracellular conditions.
Performed co-immunoprecipitation, immunoblotting, and in vitro overlay assays to assess physical and functional interactions between calponin, MAPK, and protein kinase C-epsilon.
Tracked the spatial redistribution of calponin and associated kinases using high-resolution digital confocal microscopy.
Phenylephrine triggered smooth muscle contraction in the absence of extracellular calcium without increasing intracellular ionized calcium or myosin light chain phosphorylation, but while activating MAPK.
Calponin physically associated with both MAPK and protein kinase C-epsilon via co-immunoprecipitation, although MAPK did not phosphorylate calponin in vitro.
Confocal imaging demonstrated that calponin translocated to the cell membrane alongside MAPK and protein kinase C-epsilon during phenylephrine stimulation.

Structured PICO

Population

Freshly isolated ferret aorta cells

Intervention

Phenylephrine stimulation in the absence of extracellular calcium

Outcome

Smooth muscle contraction, intracellular calcium levels, myosin light chain phosphorylation, and protein interactions (MAPK, calponin, PKC-epsilon)surrogate

Calponin may function as an adapter protein linking MAPK and PKC-epsilon to the plasmalemma during calcium-independent vascular smooth muscle contraction.

Cite This Study

Menice et al. (1997) studied Vascular smooth muscle contraction. Phenylephrine stimulation was evaluated on Smooth muscle cell contraction and protein interactions. Phenylephrine induces contraction of ferret aorta cells without increasing intracellular calcium, accompanied by MAPK activation and calponin redistribution to the cell membrane.

synapsesocial.com/papers/6a16f5104f6063e06b052040 https://doi.org/10.1074/jbc.272.40.25157

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Implication

Challenges Ca2+-dependent contraction paradigm in vascular smooth muscle; hypothesis-generating in animal models, requiring human validation.

Calponin and Mitogen-activated Protein Kinase Signaling in Differentiated Vascular Smooth Muscle

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Calponin and Mitogen-activated Protein Kinase Signaling in Differentiated Vascular Smooth Muscle

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider