What is the clinical evidence from this study?

Study design: Other. Population: Healthy rat muscle. Intervention: Maximal isometric twitch and tetanic contractions. Primary outcome: Phosphagen cost of maximal isometric twitch and tetanic contractions.

What does this research mean for the field?

The average energy cost of maximal isometric twitch contractions in rat gastrocnemius muscle is 0.257 +/- 0.012 mumol ATP/g/twitch, while tetanic contractions require 2.17 +/- 0.07 mumol ATP/g. Novelty: ClaimNovelty.CONFIRMATORY. Consensus alignment: ConsensusAlignment.SUPPORTS_CONSENSUS.

What question did this study set out to answer?

Quantify the phosphagen energy cost of maximal isometric twitch and tetanic contractions in rat skeletal muscle in situ and validate mathematical models of phosphocreatine breakdown kinetics.

January 1, 1993NMR in Biomedicine

Energy cost of twitch and tetanic contractions of rat muscle estimated in situ by gated 31P NMR

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Key result

The average energy cost of maximal isometric twitch contractions in rat gastrocnemius muscle was 0.257 +/- 0.012 mumol ATP/g/twitch, while tetanic contractions required 2.17 +/- 0.07 mumol ATP/g.

Population

Rat gastrocnemius muscle in situ

Design

Preclinical

Authors

JFJeanne M. FoleyNorthwestern University RMRonald A. MeyerMarquette University

Discussion

Member takes

Implication

Validates 31P NMR fitting method for rat muscle energetics; supports consensus estimates while leaving open human translation.

Key Points

Quantify the phosphagen energy cost of maximal isometric twitch and tetanic contractions in rat skeletal muscle in situ and validate mathematical models of phosphocreatine breakdown kinetics.
Measured phosphorus metabolites in rat gastrocnemius muscle in situ using 31P NMR gated to precise time points following brief (< 10 s) contraction bouts.
Tested twitch contractions across four stimulation rates (1 to 8 Hz) and tetanic contractions at 100 Hz (100 ms duration).
Monitored intracellular pH changes and compared initial twitch costs to time zero derivatives from exponential fits of extended contraction data.
Twitch stimulation frequency did not alter energy cost per contraction, yielding a pooled average cost of 0.257 ± 0.012 µmol ATP/g/twitch across 1 to 8 Hz.
Tetanic contractions required 2.17 ± 0.07 µmol ATP/g muscle/contraction.
Intracellular pH stability confirmed that phosphocreatine hydrolysis accounted for all ATP consumed during brief twitches, followed by a transient delay before oxidative recovery began.

Structured PICO

Population

Rat gastrocnemius muscle in situ

Intervention

Maximal isometric twitch and tetanic contractions

Outcome

Phosphagen cost (ATP use) per contraction measured by 31P NMRsurrogate

The study validates the use of the fitting/derivative method to assess energy cost in rat muscle contractions using 31P NMR.

Cite This Study

Foley et al. (1993) studied Healthy rat muscle. Maximal isometric twitch and tetanic contractions was evaluated on Phosphagen cost of maximal isometric twitch and tetanic contractions. The average energy cost of maximal isometric twitch contractions in rat gastrocnemius muscle was 0.257 +/- 0.012 mumol ATP/g/twitch, while tetanic contractions required 2.17 +/- 0.07 mumol ATP/g.

synapsesocial.com/papers/6a173dc992a42ac3ac2c39af https://doi.org/10.1002/nbm.1940060106

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Energy cost of twitch and tetanic contractions of rat muscle estimated in situ by gated 31P NMR

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Energy cost of twitch and tetanic contractions of rat muscle estimated in situ by gated 31P NMR

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider