An Ultraviolet-visible Spectrophotometric Approach to Establish a Method for Determining the Presence of Fluorescein Sodium Dye

This research look over the application of adsorbent as bentonite. Bentonite, aluminum phyllosilicate clay made up of montmorillonite. Montmorillonite known for enormous water absorption and high surface area. Dyeing industries are crucial ecological hazards donating upto twenty percent of industrial water pollution. These industries discharge toxic sewer water containing heavy metals, mutagenic substances, carcinogenic amines, and non-biodegradable chemicals, affects human health and aquatic ecosystems. The contamination of water with toxic dyes poses serious threats to surrounding. Therefore it is mandatory to overcome the environmental challenges with reliable and ability of component and environmental impact. Bentonite having capability to detoxified the harmful substances. It has the dormant for sorption the pesticide endrin possibly due to a combination of hydrophobic and charge-dipole. This study examined the potential of bentonite as adsorbent techniques for treating fluorescein sodium dyes from aqueous solutions. Experiment is done on the particular concentration of 200 ppm and at fixed pH of 2, 4 6, 8 and 10. Results recorded by the systronics spectrophotometer for absorbance Vs wavelength at pH 2 and 10 for fixed interval of time, Absorbance Vs Time, and decolorisation of dye. The performance of bentonite regarding color removal was optimum when the initial pH was 10 for 15 minutes and under ideal circumstances, decolorisation was shown to be 99.97% at alkaline pH. At pH 2 and 4 constant absorbance is achieved by 99.99% for a time intervals of 15 minutes. At an acidic pH the absorbance gradually decreased with respect to time. The spectrophotometric data at a wavelength of 490 nm is 2.683 at pH 10, shows the absorbance of toxic substances. As the wavelength is gradually increased the absorbance is also increased. The response of adsorbent as bentonite for the removal of fluorescein dyes from aqueous solution as well as for healthy environment is excellent and verified.