Key result
Blockers of the K+/Cl- cotransporter (DIOA), Cl- channel (NPPB), and K+ channel (quinine) significantly diminished the proliferation of MC3T3-E1 osteoblastic cells.
p-value: p=<0.025
Both increases and decreases in intracellular Cl- concentration diminish the proliferation of MC3T3-E1 osteoblastic cells.
In the preset report, we studied if blockers of the K+/Cl- cotransporter, and Cl- and K+ channels modify the proliferation of MC3T3-E1 osteoblastic cells. A blocker of the K+/Cl- cotransporter, DIOA (100 and 500 microM), diminished the cell proliferation. NPPB (100 and 300 microM, a blocker of Cl- channel) and quinine (100 and 500 microM, a blocker of K+ channel) also suppressed the proliferation of MC3T3-E1 cells. These blockers of the K+/Cl- cotransporter/channels (DIOA, NPPB and quinine) would increase the intracellular Cl- concentration ([Cl-]i). On the other hand, our previous study (Biochem. Biophys. Res. Commun. 361, 1038-1043, 2007) reported that a decrease in [Cl-]i diminished the proliferation of MC3T3-E1 osteoblastic cells. Taken together, the observations indicate that both increases and decreases in [Cl-]i diminish the proliferation of MC3T3-E1 osteoblastic cells.
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Maki et al. (2009) studied Osteoblast proliferation. K+/Cl- transporter and channel blockers (DIOA, NPPB, quinine) vs. Solvent control (DMSO) was evaluated on Cell proliferation (absorbance at 492 nm) (p=<0.025). Blockers of the K+/Cl- cotransporter (DIOA), Cl- channel (NPPB), and K+ channel (quinine) significantly diminished the proliferation of MC3T3-E1 osteoblastic cells.
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