What question did this study set out to answer?

To develop and validate a sensitive immunoradiometric assay for measuring circulating growth-differentiation factor 15 (GDF15) and characterize reference concentrations in healthy elderly individuals and patients with chronic heart failure.

December 22, 2006Clinical ChemistryOpen Access

Circulating Concentrations of Growth-Differentiation Factor 15 in Apparently Healthy Elderly Individuals and Patients with Chronic Heart Failure as Assessed by a New Immunoradiometric Sandwich Assay

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Authors

TKTibor KempfBoston University RHRüdiger Horn-WichmannUniversity of Alberta GBGeorg BrabantUniversity of Lübeck

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Implication

Observational assay validation reveals elevated circulating GDF15 levels linked to disease severity in chronic heart failure patients, highlighting its biomarker utility.

Key Points

To develop and validate a sensitive immunoradiometric assay for measuring circulating growth-differentiation factor 15 (GDF15) and characterize reference concentrations in healthy elderly individuals and patients with chronic heart failure.
Developed an immunoradiometric sandwich assay (IRMA) using affinity-purified polyclonal anti-human GDF15 IgG antibodies and evaluated analytical sensitivity, specificity, and preanalytic stability.
Quantified circulating GDF15 concentrations in 429 apparently healthy elderly individuals and 153 patients with chronic heart failure (CHF).
The IRMA exhibited a detection limit of 20 ng/L, intraassay imprecision ≤10.6%, interassay imprecision ≤12.2%, no cross-reactivity with TGF-β, and stability across 4 freeze-thaw cycles and 48 hours at room temperature.
Healthy elderly participants had a median GDF15 concentration of 762 ng/L (25th–75th percentiles: 600–959 ng/L), which correlated with age, cystatin C, and C-reactive protein levels.
Patients with chronic heart failure exhibited increased circulating GDF15 concentrations that correlated closely with clinical disease severity.

Cite This Study

Kempf et al. (2006) studied this question.

synapsesocial.com/papers/6a17e77756b3e2ada412c71c https://doi.org/10.1373/clinchem.2006.076828

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Implication

Observational assay validation reveals elevated circulating GDF15 levels linked to disease severity in chronic heart failure patients, highlighting its biomarker utility.

Circulating Concentrations of Growth-Differentiation Factor 15 in Apparently Healthy Elderly Individuals and Patients with Chronic Heart Failure as Assessed by a New Immunoradiometric Sandwich Assay

Discussion

Member takes

Implication

Key Points

Cite This Study

Circulating Concentrations of Growth-Differentiation Factor 15 in Apparently Healthy Elderly Individuals and Patients with Chronic Heart Failure as Assessed by a New Immunoradiometric Sandwich Assay

Discussion

Member takes

Implication

Key Points

Cite This Study

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