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January 1, 1985Nucleic Acids ResearchOpen Access

The use of phosphorothioate-modified DNA in restriction enzyme reactions to prepare nicked DNA

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Authors

John W. Taylor
John W. TaylorNew Mexico State UniversityWSWalter SchmidtAFFiRiS (Austria)RCRichard CosstickUniversity of Liverpool

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Taylor et al. (1985) studied this question.

synapsesocial.com/papers/6a183d31aeefdf6d9c138809https://doi.org/10.1093/nar/13.24.8749
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Also Consider

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  1. 1Synthesis and characterization of an octanucleotide containing the EcoRI recognition sequence with a phosphorothioate group at the cleavage site1984 · 159 citations
  2. 2Synthesis and properties of diastereoisomers of adenosine 5'-(O-1-thiotriphosphate) and adenosine 5'-(O-2-thiotriphosphate)1976 · 219 citations
  3. 3Studies on Sequence Recognition By Type II Restriction and Modification Enzyme1982 · 119 citations
  4. 4The SalGI restriction endonuclease. Mechanism of DNA cleavage1982 · 35 citations
  5. 5Local mutagenesis: a method for generating viral mutants with base substitutions in preselected regions of the viral genome.1978 · 284 citations