What does this research mean for the field?

During adenovirus 2 replication, the 10-fold increase in endogenous RNA polymerase II and III activities is not caused by increased cellular concentrations of these enzymes, but likely results from increased viral DNA template availability or functional modifications. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

To determine whether increases in viral RNA synthesis during adenovirus 2 infection stem from changes in the concentration or activity of host DNA-dependent RNA polymerases.

January 1, 1976Journal of VirologyOpen Access

Viral RNA Synthesis and Levels of DNA-Dependent RNA Polymerases During Replication of Adenovirus 2

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Population

Cultured human KB cells infected by adenovirus 2

Comparison

Adenovirus 2 infection vs Mock-infected cells

Design

Preclinical

Authors

RWRoberto WeinmannThe Wistar Institute JJJudith A. JaehningUniversity of Colorado Denver HRHeschel J. RaskasState University of New York

Discussion

Member takes

Implication

Indicates infection enhances nuclear polymerase activity independently of enzyme abundance; leaves open roles in viral pathogenesis.

Key Points

To determine whether increases in viral RNA synthesis during adenovirus 2 infection stem from changes in the concentration or activity of host DNA-dependent RNA polymerases.
Infected cultured human KB cells with adenovirus 2 and measured endogenous RNA polymerase I, II, and III activities in isolated nuclei using alpha-amanitin sensitivity.
Quantitated viral mRNA precursor synthesis via polyacrylamide gel electrophoresis and assayed total cellular enzyme levels with exogenous templates following DEAE-Sephadex chromatographic resolution.
Endogenous nuclear RNA polymerase II and III activities increased approximately 10-fold alongside viral mRNA precursor synthesis, whereas RNA polymerase I activity remained constant.
Total solubilized cellular levels and chromatographic properties of RNA polymerases I, II, and III remained unchanged throughout infection.

Structured PICO

Population

Cultured human KB cells infected by adenovirus 2

Intervention

Adenovirus 2 infection

Comparator

Mock-infected cells

Outcome

Rates of RNA synthesis and levels of RNA polymerases I, II, and IIIsurrogate

The increase in endogenous RNA polymerase activities during adenovirus 2 infection is likely due to increased viral DNA template availability or functional modifications rather than increased cellular concentrations of the enzymes.

Cite This Study

Weinmann et al. (1976) studied this question.

synapsesocial.com/papers/6a1863f5a61bfdbaa2c995b0 https://doi.org/10.1128/jvi.17.1.114-126.1976

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January 1, 1976Journal of VirologyOpen Access

Viral RNA Synthesis and Levels of DNA-Dependent RNA Polymerases During Replication of Adenovirus 2

View Full Paper

Ask AI

Bookmark

Population

Cultured human KB cells infected by adenovirus 2

Comparison

Adenovirus 2 infection vs Mock-infected cells

Design

Preclinical

Authors

RWRoberto WeinmannThe Wistar Institute JJJudith A. JaehningUniversity of Colorado Denver HRHeschel J. RaskasState University of New York

Discussion

Member takes

Implication

Indicates infection enhances nuclear polymerase activity independently of enzyme abundance; leaves open roles in viral pathogenesis.

Key Points

To determine whether increases in viral RNA synthesis during adenovirus 2 infection stem from changes in the concentration or activity of host DNA-dependent RNA polymerases.
Infected cultured human KB cells with adenovirus 2 and measured endogenous RNA polymerase I, II, and III activities in isolated nuclei using alpha-amanitin sensitivity.
Quantitated viral mRNA precursor synthesis via polyacrylamide gel electrophoresis and assayed total cellular enzyme levels with exogenous templates following DEAE-Sephadex chromatographic resolution.
Endogenous nuclear RNA polymerase II and III activities increased approximately 10-fold alongside viral mRNA precursor synthesis, whereas RNA polymerase I activity remained constant.
Total solubilized cellular levels and chromatographic properties of RNA polymerases I, II, and III remained unchanged throughout infection.

Structured PICO

Population

Cultured human KB cells infected by adenovirus 2

Intervention

Adenovirus 2 infection

Comparator

Mock-infected cells

Outcome

Rates of RNA synthesis and levels of RNA polymerases I, II, and IIIsurrogate

Cite This Study

Weinmann et al. (1976) studied this question.

synapsesocial.com/papers/6a1863f5a61bfdbaa2c995b0 https://doi.org/10.1128/jvi.17.1.114-126.1976

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

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