Ferritin Synthesis in Lymphocytes, Polymorphs and Monocytes

A rapid two-stage method has been devised for the separation of different leucocyte populations from human blood. Different cell types can be obtained in an undamaged state and with little contamination. Ferritin and total protein synthesis has been determined by measuring [14C]leucine incorporation in culture media which contain varying amounts of added ferric iron or desferrioxamine. Both ferritin and total protein synthesis is greater in monocytes than in lymphocytes or polymorphs when the basal medium is used. Only monocytes show a consistent increase in ferritin production due to iron stimulation. Ferritin synthesis by monocytes, polymorphs and lymphocytes is inhibited in the presence of desferrioxamine.