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July 1, 1994Cardiovascular Research

Adenosine receptor specificity in preconditioning of isolated rabbit cardiomyocytes: evidence of A3 receptor involvement

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Why the study?

Does activation of specific adenosine receptor subtypes (A3) induce preconditioning and reduce ischaemic injury in isolated rabbit cardiomyocytes?

Population

Isolated rabbit cardiomyocytes

Comparison

5 min preincubation in the presence or absence… vs Control conditions

Design

Preclinical

Authors

SAS ArmstrongBeth Israel Deaconess Medical CenterCGCharles E. GanoteNorthwestern University

Discussion

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Implication

A3 receptor involvement may mediate cardiomyocyte preconditioning; hypothesis-generating in animals and leaves open human translation.

Structured PICO

Does activation of specific adenosine receptor subtypes (A3) induce preconditioning and reduce ischaemic injury in isolated rabbit cardiomyocytes?

P
Population
Isolated rabbit cardiomyocytes
I
Intervention
5 min preincubation in the presence or absence of glucose and various agonists and antagonists of adenosine receptors (e.g., R-PIA, CGS 12680, APNEA, SPT, DPCPX, BW 1433U83, Calphostin C)
C
Comparator
Control conditions (e.g., presence of glucose, absence of specific agonists/antagonists)
O
Outcome
Rate and extent of injury during ischaemia (assessed by trypan blue permeability following 85 mOsm swelling)surrogate

Preconditioning in isolated rabbit cardiomyocytes appears to require participation of adenosine A3 receptors and is likely mediated by protein kinase C activation.

Cite This Study

Armstrong et al. (1994) studied this question.

synapsesocial.com/papers/6a19902e60e90a7f5fea670ahttps://doi.org/10.1093/cvr/28.7.1049
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1In vitro ischaemic preconditioning of isolated rabbit cardiomyocytes: effects of selective adenosine receptor blockade and calphostin C1995 · 46 citations
  2. 2Evidence that the adenosine A3 receptor may mediate the protection afforded by preconditioning in the isolated rabbit heart1994 · 278 citations
  3. 3Preconditioning of isolated rabbit cardiomyocytes: effects of glycolytic blockade, phorbol esters, and ischaemia1994 · 61 citations
  4. 4Preconditioning of isolated rabbit cardiomyocytes: induction by metabolic stress and blockade by the adenosine antagonist SPT and calphostin C, a protein kinase C inhibitor1994 · 156 citations
  5. 5Adenosine and A1 selective agonists offer minimal protection against ischaemic injury to isolated rat cardiomyocytes1993 · 32 citations