Role of superoxide dismutase in in vivo and in vitro nitrate tolerance
Population
Endothelium-intact aortic rings from rabbits (in vivo tolerant and control)
Comparison
In vitro treatment with GTN, tiron, or… vs Control aortic rings
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Distinguishes in vivo from in vitro nitrate tolerance mechanisms; leaves open clinical translation.
Structured PICO
P
Population
Endothelium-intact aortic rings from rabbits (in vivo tolerant and control)
I
Intervention
In vitro treatment with GTN (1 h 10 microM), tiron (10 mM), or diethyldithiocarbamate (DETC; 10 mM)
C
Comparator
Control aortic rings
O
Outcome
Vasorelaxant response to GTN and acetylcholine, superoxide radical formation, NADH oxidase activity, and SOD activity/expressionsurrogate
In vivo nitrate tolerance is driven by increased superoxide production via NADH oxidase activation and SOD inactivation, distinct from the mechanism of in vitro tolerance.
Cite This Study
Münzel et al. (1999) studied this question.